A　novel　method　for　fast　determination　of　nicotine　in　tobacco　and　cigarette　was　established　by　using　high　performance　liquid　chromatography　(HPLC)　coupled　with　a　partly　immersed　three　phase　hollow　fiber　liquid　phase　microextraction　(PILPME)　technique　for　sample　preparation.　The　model　of　partly　immersed　was　introduced　for　the　first　time.　This　study　has　demonstrated　that　PILPME　constituted　a　real　alternative　to　the　other　liquid　phase　microextraction　methods.　Parameters　related　to　PILPME　(organic　solvent,　pH　of　donor　and　acceptor　phase,　the　proportion　of　hollow　fiber　in　gas　and　liquid,　extraction　time,　temperature)　were　optimized　experimentally,　for　preconcentration　and　extraction　of　nicotine　in　tobacco　and　cigarette　samples　has　shown　many　advantages　over　traditional　sample　preparation　technique,　which　provided　a　good　linear　range　(0.05-10　mu　g/mL)　with　r(2)　=　0.9996,　a　low　detection　limit　(5　ng/mL,　S/N　=　3)　and　a　satisfactory　relative　standard　deviation　(＜8　%).　The　result　showed　3.03　and　3.39　mg/gin　tobacco　and　1.51,　1.45　and　1.73　ing/g　in　cigarette,　respectively.　The　recovery　was　obtained　in　the　range　of　95.91-97.95　%.