Solid,　cracked,　and　flower-shaped　surfaces　of　palladium　nanospheres　with　high　yields　and　good　uniformity　were　successfully　prepared　by　a　wet　chemical　method.　On　the　basis　of　the　experimental　data,　the　same　size　of　palladium　nanosphere　with　different　surface　morphologies　can　be　regulated　only　by　changing　the　amount　of　ammonium　hydroxide　and　reductant　in　one　experimental　system.　The　as-prepared　products　were　studied　by　transmission　electron　microscopy　(TEM),　scanning　electron　microscopy　(SEM)　and　x-ray　diffraction　(XRD).　In　addition,　surface-enhanced　Raman　scattering　(SERS)　spectra　on　the　as-prepared　different　surface　of　palladium　nanospheres　exhibit　high　activity　towards　p-aminothiophenol　(PATP)　detection,　and　the　result　further　reveals　that　the　predominance　of　the　a1　vibration　mode　in　the　SERS　spectra　via　an　electromagnetic　(EM)　mechanism　is　significant.