Objective:　Functional　neuroimaging　techniques　act　as　the　navigator　to　assess　changes　in　brain　activity　induced　by　repetitive　transcranial　magnetic　stimulation　(rTMS)　in　rTMS　studies.　The　aim　of　this　study　was　to　investigate　the　feasibility　of　using　manganese-enhanced　magnetic　resonance　imaging　(MEMRI)　to　measure　the　brain　activity　in　rTMS　studies.　Methods:　Eighteen　Wistar　rats　were　randomized　into　three　groups　(n=6)　including　a　high　rTMS　group,　a　low　rTMS　group　and　a　sham　stimulation　group　(controls).　They　were　given　rTMS　of　10　Hz,　1　Hz　or　sham　stimulation　for　5　hours　separately.　MEMRI　was　used　to　assess　the　changes　of　brain　activity.　Results:　Compared　with　the　controls,　image　intensity　was　enhanced　differently　in　various　brain　regions　on　T1-weighted　images　after　rTMS　with　different　frequencies,　higher　intensity　and　wider　enhancement　occurred　in　the　high　frequency　rTMS　group　as　compared　with　that　in　the　low　frequency　rTMS　group.　Conclusion:　MEMRI　can　be　used　to　reveal　the　changes　of　brain　activity　in　live　rats　following　rTMS.　Significance:　The　current　experiment　might　provide　a　new　functional　neuroimaging　technique　for　the　study　of　rTMS.