A　solid　phase　extraction-high　performance　liquid　chromatographic　(SPE-HPLC)　method　was　developed　for　the　determination　of　phthalic　acid　esters　and　their　metabolites　in　human　urine.　The　conditions　of　pretreatment　such　as　eluent,　elution　volume　and　elution　velocity　were　optimized.　The　optimized　conditions　were　2　mL　of　acetonitrile,　2　mL　of　ethyl　acetate　and　1　mL　of　ethyl　ester-n-hexane　(1/19,　V/V)　at　the　flow　rate　of　1　mL/min.　Under　the　optimized　conditions,　five　phthalic　acid　esters　and　their　monoesters　were　determined　by　reversed　phase　liquid　chromatography.　Good　linear　relationships　were　obtained　in　the　range　of　20　mu　g/L　to　10000　mu　g/L　with　the　correlation　coefficients　above　0.　99.　The　limits　of　detection　were　between　3.　8　mu　g/L　and　7.　0　mu　g/L.　The　recoveries　were　in　the　range　of　72.　2%　to　102.　3%　and　relative　standard　deviations　were　lower　than　8%.　The　method　was　applied　to　analyze　five　human　urines　collected　from　five　fertile　women.　Except　for　dimethyl　phthalate　and　diethyl　phthalate,　the　others　were　found　in　the　urines.