The　activatable　off-on　near-infrared　QCy7-based　fluorogenic　probes　have　emerged　as　powerful　modalities　for　detecting　and　monitoring　biological　analytes　and　understanding　their　biological　processes　in　cells　and　organisms.　The　use　of　biomarker-activated　QCy7-based　probes　enables　simple　synthesis,　minimum　photo-damage　to　biological　samples,　and　minimum　background　interference　from　biological　systems.　In　this　minireview,　we　aim　to　provide　a　rigorous　but　concise　overview　of　activatable　QCy7-based　fluorogenic　probes　by　reporting　the　significant　progress　made　in　recent　years.　The　design　strategies　and　the　main　applications　of　accurate　detection　and　imaging　of　disease-related　biomarkers　(including　ROS/RSS,　enzymes,　metal　ions,　and　other　related　species)　were　reasonably　analyzed　and　discussed.　The　potential　challenges　and　prospects　of　activatable　QCy7-based　fluorogenic　probes　are　also　emphasized　to　further　advance　the　development　of　new　methods　for　biomarker　detection　and　bioimaging.