A　non-invasive　detection　method　for　the　status　analysis　of　cell　culture　is　presented　based　on　digital　holography　technology.　Lens　less　Fourier　transform　digital　holography　(LFTDH)　configuration　is　developed　for　living　cell　imaging　without　prestaining.　Complex　amplitude　information　is　reconstructed　by　a　single　inverse　fast　Fourier　transform,　and　the　phase　aberration　is　corrected　through　the　two-step　phase　subtraction　method.　The　image　segmentation　is　then　applied　to　the　automatic　evaluation　of　continency.　Finally,　the　cervical　cancer　cell　TZMbl　is　employed　for　experimental　validation,　and　the　results　demonstrate　that　LFTDH　imaging　with　the　corresponding　image　post-processing　can　provide　an　automatic　and　non-invasive　approach　for　monitoring　living　cell　culture.