Some　polycyclic　aromatic　hydrocarbons　(PAHs)　are　typical　promutagens　that　require　metabolic　activation　to　exhibit　their　mutagenicities　and　carcinogenicities.　The　metabolites　of　three　PAHs,　pyrene　(PY),　fluoranthene　(FLU),　and　benzo[a]pyrene　(BaP),　produced　from　the　peroxynitrite/T(p-Cl)PPFeCl(peroxynitrite/(chloride)iron(III)tetrakis(p-chlorophenyl)porphyrin)　system,　have　been　identified　with　high-performance　liquid　chromatography　coupled　with　electron　spray　ionization　tandem　mass　spectrometry.　The　results　demonstrated　that　three　major　metabolites　were　the　quinone　group,　OH　group,　and　nitro　group.　In　the　Ames　test,　all　three　PAH　metabolites　became　mutagenic　without　using　the　enzymatic　activating　system,　whereas　their　parents　did　not　show　positive　results.　Cell　transformation　assay　indicated　that　I,3-nitro-BaP　and　BaP　metabolites　produced　from　this　biomimetic　system　have　more　serious　effects　in　inducing　cancer　than　the　BaP　parent.　Environ.　Toxicol.　Chem.　2011;30:723-729.　(C)　2010　SETAC