Brain　tumor　stem-like　cells　(BTSLCs)　have　been　implied　to　play　an　important　role　in　genesis　and　development　of　glioma.　However,　their　characteristics　on　proliferation　and　drug-resistance　are　uncertain　thoroughly.　In　this　experiment,　some　of　the　biological　characteristics　about　BTSLCs　were　explored.　Twenty　cases　of　different　grades　of　human　glioma　tissues　were　obtained　from　clinic.　The　primary　glioma　cells　were　collected　and　CD133(+)　cells　from　them　were　purified　by　magnetic　cell　sorting　assay.　The　BTSLCs　were　identified　by　testing　the　expression　of　CD133,　Nestin,　NSE,　and　GFAP,　along　with　the　culture　process.　WST-8　assay　kit　was　used　to　evaluate　the　proliferating　situation　of　CD133(+)　cells　in　the　different　grade　gliomas,　and　to　compare　the　drug-resistance　between　the　CD133(+)　and　CD133　(-)　cells　in　the　medium　containing　different　concentrations　of　teniposide　(VM-26).　The　results　showed　that　the　CD133(+)　cells　could　regenerate　by　self-renewal,　then　generate　and　different　into　NSE(+)　and　GFAP(+)　cells,　respectively.　CD133(+)　cells　in　the　high　grade　of　gliomas　showed　the　faster　generation　than　the　ones　in　the　low　grade.　The　number　of　survived　CD133(+)　cells　in　the　medium　containing　VM-26　was　much　more　than　the　CD133(-)　ones　in　it.　Therefore,　it　was　implied　that　the　CD133(+)　BTSLCs　existed　in　the　glioma　tissues　possessed　the　more　tolerant　ability　to　the　VM-26,　and　could　proliferate　much　more　easily　in　the　high-grade　glioma.