Recent　studies　show　that　IL-13R　alpha　2,　a　brain　tumor-associated　antigen　for　IL-13,　may　play　a　role　in　immunotherapy　for　glioblastoma　Thus,　we　stimulated　the　lymphocyte　by　monocyte-derived　dendritic　cells　(DCs)　The　DCs　were　pulsed　with　IL-13R　alpha　2　in　vitro　and　then　co-cultured　with　lymphocytes　After　inducing　cytotoxic　T　cells　(CTLs)　and　co-culturing　with　U-251　cells　for　24　h　in　96　wells,　Cell　Count　Kit-8　(CCK-8)　was　added　to　every　well　equally　The　optical　density　(OD)　value　was　detected　and　recorded　after　2　Is　The　DCs　efficiently　presented　the　antigen　to　the　CTLs,　resulting　in　CTLs　activation　and　proliferation　The　induced　CTLs　showed　specific　cytotoxic　against　U-251　cells　(P　＜　0　01)　The　results　demonstrated　that　IL-13R　alpha　2　induced　CTLs　could　kill　glioma　U-251　in　vitro,　which　suggests　that　IL-13　R　alpha　2　might　have　such　an　impact　in　vivo　and　thus　recombinant　IL-13Ra2　protein　might　be　used　as　an　anti-tumor　vaccine,　providing　a　promising　new　strategy　for　the　treatment　of　brain　malignant　gliomas　Crown　Copyright　(C)　2010　Published　by　Elsevier　Inc　All　rights　reserved.