Based　on　the　structure　of　the　HIV　integrase　core　domain,　dipeptide　derivatives,　as　a　type　of　HIV　integrase　inhibitor,　were　synthesized,　and　their　fragmentation　pathways　were　investigated　by　electrospray　ionization　mass　spectrometry　(ESI-MSn)　in　conjunction　with　tandem　mass　spectrometry　(MS/MS).　In　order　to　better　understand　the　fragmentation　pathways,　the　MS2　and　MS3　spectra　of　the　title　compound　were　obtained.　The　main　fragmentation　pathways　occur　by　the　cleavage　of　the　C-CO　bonds　between　N-(benzothiazol-2-yl)aminocarbonyl　and　methylene,　NH-CO　bonds　between　the　NH　groups　and　carbonyl　groups.　Electrospray　ionization　was　proven　to　be　a　good　method　for　the　structural　characterization　and　identification　of　this　kind　of　compound.