Au-Pt　bimetallic　nanoparticles　film　used　as　an　efficient　electrochemical　sensor　was　Prepared　by　self-assembled　Au-Pt　bimetallic　nanoparticles　on　it　glassy　carbon　(GC)　Substrate　using　thioglycolic　acid　as　a　linker.　Atomic　force　microscopy　(AFM)　and　scanning　electron　microscopy　(SEM)　showed　that　the　Au-Pt　nanoparticles　self-assembly　film　was　dense　and　uniform.　Electrochemical　experiments　revealed　that　Au-Pt　bimetallic　nanoparticles　film/GC　electrode　showed　high　electrocatalytic　activity　to　the　oxidation　of　nitric　oxide.