Protein　is　one　of　major　bio-functional　performers.　As　one　of　several　crucial　proteomic　research　approaches,　protein　microarray　has　these　following　advantages:　high-throughput,　high　sensitivity,　quick　detection　and　so　on.　Meanwhile,　there　are　some　critical　factors　that　are　important　to　the　further　development　of　protein　microarray　technology,　for　example,　how　to　express　and　purify　proteins　for　the　research　of　protein　microarray,　how　to　immobilize　proteins　onto　the　substrate　and　keep　the　bio-function　of　proteins　immobilized.　Nano-biotechnology　and　cell-free　expression　system　have　been　used　to　fabricate　protein　microarray　by　the　way　of　immobilizing　target　genes　onto　the　substrate　and　directly　expressing　corresponding　proteins,　which　provides　a　new　strategy　to　fabricate　more　complicated　microarray.　The　stragegy　and　its　progress　were　summarized-fabrication　of　protein　microarray　based　on　DNA,　including　immobilization　of　target　genes,　cell-free　expression　to　proteins,　immobilization　of　renascence　proteins,　advantages　and　drawbacks　of　the　methods　of　protein　chip　fabrication　etc.