Despite　advances　in　surgical　technology　and　radiation　therapy,　the　prognosis　in　the　patients　with　malignant　glioma　remains　poor.　Recent　studies　show　that　interleukin-13　receptor　{alpha}2　chain　(IL-13Ra2),　a　brain　tumor-associated　receptor　for　IL-13,　may　play　a　role　in　immunotherapy　for　glioblastoma.　We　thus　amplified　human　IL-13Ra2　gene　from　the　human　glioblastoma　cell　line　using　RT-PCR　and　cloned　the　target　gene　into　the　pET-28a,　a　prokaryotic　expressing　plasmid.　After　transformation,　the　recombinant　plasmid　expressed　a　soluble　protein　induced　by　IPTG.　The　purified　recombinant　protein　was　shown　to　be　a　single　band　on　the　SDS-PAGE　with　a　predicated　molecular　weight　of　human　IL-13Ra2　gene,　suggesting　that　the　recombinant　protein　of　human　IL-13Ra2　was　successfully　expressed.　Recombinant　IL-13Ra2　protein　can　be　used　as　an　anti-tumor　vaccine,　which　may　provide　a　promising　new　strategy　for　the　treatment　of　brain　malignant　gliomas.