To　study　the　immune　responses　elicited　by　multiple　vectors　and　develop　vaccines　strategies　against　prevalent　HIV-1　strains　in　China,　we　have　examined　the　potency　of　vaccine　regimens　of　plasmid　DNA,　adenovirus,　and　Sendai　virus　vectors　expressing　HIV-1　gag　consensus　sequence　of　HIV-1　isolates　from　China　for　inducing　specific　immune　responses.　In　BALB/c　mice,　combination　of　these　vectors　induced　higher　Gag-specific　cellular　immune　response　than　any　regimen　using　single　vector　alone.　The　prime-boost-boost　regimen　consisting　of　the　triple　heterologous　vectors　induced　Gag-specific　T-cell　responses　the　most　efficiently.　In　rhesus　macaques,　the　prime-boost-boost　regimen　induced　potent　Gag-specific　cellular　immune　responses　as　well　as　long　lasting　humoral　immune　response,　and　each　booster　resulted　in　rapid　and　efficient　expansion　of　Gag-specific　T　cells.　These　results　indicate　that　this　prime-boost-boost　regimen　using　triple　heterologous　vectors　is　a　promising　AIDS　vaccine　candidate　for　efficiently　inducing　HIV-1-specific　cellular　and　humoral　immune　responses.　Its　further　studies　as　a　promising　scheme　for　therapeutic　and/or　prophylactic　HIV-1　vaccines　should　be　grounded.　(C)　2008　Elsevier　Ltd.　All　rights　reserved.