The　feasibility　of　simultaneous　partial　nitrification,　denitrification　and　phosphorus　removal　(SPNDPR)　process　was　investigated　in　a　single-stage　anaerobic/micro-aerobic　sequencing　batch　reactor　for　treating　real　sewage.　Partial　nitrification　was　maintained　with　average　nitrite　accumulation　ratio　of　90.3%　during　266　days＇　operation.　Removal　efficiencies　for　NH4+-N　(96.3%),　total　inorganic　nitrogen　(81.4%),　and　phosphorus　(91.0%)　were　stably　obtained　when　treated　real　sewage　with　low　carbon/nitrogen　(3.4),　with　simultaneous　partial　nitrification　and　denitrification　efficiency　of　73.1%.　The　mechanism　analysis　revealed　that　denitrifying　glycogen-accumulating　organisms　(DGAOs)　and　denitrifying　polyphosphate-accumulating　organisms　(DPAOs)　played　the　main　roles　in　N-removal　and　P-removal,　respectively.　Nitrite　pathway　and　optimized　use　of　the　organic　carbon　available　in　the　sewage　were　keys　for　the　successful　performance.　Further　microbial　community　illustrating　that　DGAOs　Candidatus_Competibacter,　DPAOs　Dechloromonas,　and　ammonia-oxidizing　bacteria　Nitrosomonadaceae　were　main　functional　groups.　Notably,　sludge　granulation　was　formed　under　long-term　synchronous　low　dissolved　oxygen　and　low　sludge　loading　conditions,　avoiding　sludge　bulking.　(C)　2020　Elsevier　Ltd.　All　rights　reserved.