Fungi　can　be　used　in　a　bio-trickling　filter　(BTF)　to　treat　volatile　organic　compound　pollutants.　To　explore　the　effect　of　liquid　supply　on　the　performance　of　a　fungal　BTF,　a　three-layer　BTF　filled　with　ceramsite　and　inoculated　with　Fusarium　oxysporum　to　treat　toluene　was　constructed.　The　BTF　started　rapidly　(＜　10　days)　and　could　be　restarted　within　4　days　after　24　day＇　stagnation.　High-throughput　sequencing　showed　that　Fusarium　evolved　into　a　complex　microbial　system.　The　middle　layer　had　the　most　even　proportions　of　dominant　fungi　and　the　highest　coexistence　of　various　genera.　The　effect　of　liquid　supply　on　the　performance　of　microorganism　in　the　BTF　was　investigated　in　detail.　The　pH　of　the　nutrient　liquid　fluctuated　between　5.6　and　8.1.　Small　amounts　of　nutrient　sprayed　at　frequent　intervals　(49　mL　every　2　h)　and　a　minimum　humidity　of　40　%　provided　optimal　conditions　for　fungal　survival.　Nutrient　replacement　cycle　can　be　extended　from　one　week　to　two　weeks,　and　timely　removal　of　microelements　can　enhance　the　microbial　activity.　Moreover,　fungi　can　grow　well　at　ambient　temperatures　of　16　to　28　degrees　C.