Surface　Enhanced　Raman　Scattering　(SERS)　combined　with　Thin　Layer　Chromatography　(TLC)　has　been　used　for　studying　characteristic　spectrum　of　molecules　in　situ　in　micrograms　samples.　There　are　very　few　report　for　applying　the　SERS-TCL　method　in　the　study　of　the　effective　ingredients　of　Chinese　traditional　herbs.　Coptis　Chinensis　France　is　an　often-used　clinic　Chinese　traditional　medicine.　Its　main　effective　components　include　berberine　and　so　on,　which　have　antibiotic　very　wide　and　also　have　curative　effect　on　improving　the　functions　of　heart　vascular　cycles.　Therefore　the　concentrations　of　berberine　are　very　important　for　the　quality　control　of　the　medicine.　In　this　work,　the　ethanol　extract　of　Coptis　Chinensis　France　was　first　separated　by　TLC,　the　SERS　was　then　measure　directly　after　dropping　silver　gel　on　the　separated　spots.　The　method　can　be　used　for　the　finger　print　analysis　of　the　berberine.　3　muL　of　alcohol　extract　of　Coptis　Chinensis　Franch　with　total.　alkaloids　concentration　of　1.0　mg　.　mL　was　placed　on　silicon　GF(254)TLC　plate.　The　sample　was　separated　by　developing　solvent　of　n　bulanol-Acitic　acid-H2O　(7:2:　1　V/V).　The　positions　of　berberine　in　the　sample　were　confirmed　by　the　standard　alkaloid　solutions.　The　Rf　values　for　berberine　are　0.29.　The　silver　gel　was　used　as　surface　enhanced　substrate　and　placed　on　the　separated　berberine　spots.　FT-SERS　was　measured　directly　by　a　Nicolet　FT-Raman　910　spectrometer.　Berberine　belong　to　isoquinoline　alkaloids.　His　structure　can　be　found　in　reference.　The　date　of　spectrum　of　berberine　can　be　seen　that　the　band　at　1396　cm(-1)　due　to　Ar-OCH3　deformation　vibrations　was　greatly　enhanced,　indicating　that　the　molecule　was　absorbed　on　silver　gel　strongly　through　lone-pair　electron　in　An　OCH3.　The　ring　stretching　mode　occurring　around　1　548　cm(-1)　represents　isoquinoline　ring　in　the　molecule.　The　band　at　727　cm(-1)　due　to　CH　(ring)　deformation　vibrations　was　also　enhanced.