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作者:

Wang, Haiyan (Wang, Haiyan.) | Liu, Wei (Liu, Wei.) | Hao, Ruixia (Hao, Ruixia.)

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摘要:

Dietary oil spillage has become a widespread and increasing threat to the environment worldwide. Lipase produced by bacteria provides an efficient, environment-friendly and cost-saving way to degrade dietary oil waste. Thus, selection of highly efficient lipases becomes an urgent need to solve dietary oil-related environmental issues. In this study, with comparison analysis of different bacteria species showing oil degradation abilities, we found Bacillus cereus XN12 displayed higher dietary oil degradation efficiency than Bacillus subtilis and Enterobacter cloacae. Bacillus cereus flourishes better in oil-containing medium than non-oil medium, which indicated that Bacillus cereus prefers to use dietary oil as their energy source. We then successfully cloned the lipase gene from Bacillus cereus XN12 and expressed this Bacillus cereus XN12 lipase gene in Escherichia coli BL21. The Escherichia coli expressing the recombinant Bacillus cereus lipase protein displayed significant higher lipase activity and oil degradation ability. These results suggested that lipase from Bacillus cereus XN12 provides a potential solution to alleviate dietary oil pollution.

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作者机构:

  • [ 1 ] [Wang, Haiyan]Beijing Univ Technol, Key Lab Beijing Reg Air Pollut Control, Beijing 100124, Peoples R China
  • [ 2 ] [Liu, Wei]Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing 100124, Peoples R China
  • [ 3 ] [Hao, Ruixia]Beijing Univ Technol, Coll Construct Engn, Beijing 100124, Peoples R China

通讯作者信息:

  • [Hao, Ruixia]Beijing Univ Technol, Coll Construct Engn, Beijing 100124, Peoples R China

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来源 :

4TH INTERNATIONAL CONFERENCE ON ADVANCES IN ENERGY RESOURCES AND ENVIRONMENT ENGINEERING

ISSN: 1755-1307

年份: 2019

卷: 237

语种: 英文

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WoS核心集被引频次: 3

SCOPUS被引频次: 4

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