Forward　osmosis　(FO)　membrane　with　high　separation　performance　is　needed　to　promote　its　practical　applications.　Herein,　layer-by-layer　(LbL)　approach　was　used　to　prepare　a　thin　and　highly　cross-linked　polyamide　layer　on　a　polyacrylonitrile　substrate　surface　to　prepare　a　thin-film　composite　forward　osmosis　(TFC-FO)　membrane　with　enhanced　FO　performance.　The　effects　of　monomer　concentrations　and　assembly　cycles　on　the　performance　of　the　TFC-FO　membranes　were　systematically　investigated.　Under　the　optimal　preparation　condition,　TFC-FO　membrane　achieved　the　best　performance,　exhibiting　the　water　flux　of　14.4/6.9　LMH　and　reverse　salt　flux　of　7.7/3.8　gMH　under　the　pressure　retarded　osmosis/forward　osmosis　(PRO/FO)　mode　using　1M　NaCl　as　the　draw　against　a　DI-water　feed,　and　a　rejection　of　96.1%　for　2000　mg/L　NaCl　aqueous　solution.　The　result　indicated　that　layer-by-layer　method　was　a　potential　method　to　regulate　the　structure　and　performance　of　the　TFC-FO　membrane.