It　is　well　known　that　excessive　drinking　could　result　in　human　diseases　(e.g.,　liver　disease　and　cardiovascular　disease).　To　investigate　the　underlying　mechanisms　of　the　effects　of　alcohol　consumption,　dental　pulp　stem　cells　have　been　used　due　to　their　self-renewal　capability,　multi-lineage　differentiation,　and　clonogenic　efficiency.　Researchers　usually　focus　on　detecting　genes　having　different　mean　expression　levels　between　different　conditions.　However,　the　variance　of　gene　expression　might　also　provide　useful　information.　In　this　study,　we　applied　the　Brown-Forsythe　(BF)　test　for　equal　variance　based　on　a　human　dental　pulp　stem　cells　dataset　to　explore　the　role　of　alcohol　on　the　variation　of　gene　expression.　We　identified　two　differential　variable　(DV)　gene　probes,　213597_s_at　near　gene　CTDSPL　(FDR-adjusted　p-value　=　1.50x10-5)　and　213993_at　near　gene　SPON1　(FDR-adjusted　p-value=0.042).　To　best　of　our　knowledge,　no　studies　have　found　yet　the　associations　of　CTDSPL　and/or　SPON1　to　alcohol　consumption.　GeneMANIA　showed　that　these　two　genes　are　related　to　20　genes.　Web　based　gene　set　analysis　toolkit　(WebGestalt)　showed　that　these　22　genes　are　enriched　in　4　alcohol-related　KEGG　pathways:　adherens　junction,　TGF-beta　signaling　pathway,　signaling　pathways　regulating　pluripotency　of　stem　cells,　and　Hippo　signaling　pathway.