Moderate　alcohol　consumption　has　health　benefits　of　adults,　while　excessive　alcohol　has　poisonous　effects　on　numerous　different　organs.　However,　the　underlying　molecular　mechanisms　of　this　phenomenon　are　still　largely　unclear　yet.　Human　dental　pulp　stem　cells　(hDPSCs)　have　been　used　as　models　to　investigate　the　effect　of　alcohol　(EtOH)　on　gene　expression.　It　has　been　demonstrated　that　both　EtOH　dosage　and　exposure　time　affects　gene　expression.　However,　the　investigation　into　whether　there　exists　synergistic　effect　of　EtOH　dosage　and　exposure　time　on　gene　expression　is　rare.　In　the　present　study,　we　reported　that　the　effect　of　interaction　between　EtOH　dosage　and　exposure　time　is　significantly　associated　with　the　expression　of　genes　SLC25A36　and　ZFAND5　based　on　an　hDPSC　experiment.　This　result　is　novel　in　that　genes　SLC25A36　and　ZFAND5　have　not　been　reported　yet　to　be　associated　with　alcohol　consumption　in　the　existing　literature.