Antibiotic　resistance　genes　(ARGs)　and　mobile　gene　elements　(MGEs),　the　emerging　genetic　contaminants,　are　regarded　as　severe　risks　to　public　health　for　impairing　the　inactivation　efficacy　of　antibiotics.　Secondary　effluents　from　wastewater　treatment　plants　are　the　hotspots　for　spreading　these　menaces.　Herein,　sulfidated　nanoscale　zero-valent　iron　(S-nZVI)　was　occupied　to　remove　ARGs　and　MGEs　in　secondary　effluents　and　weaken　the　regrowth　capacity　of　their　bacterial　carriers.　The　effects　of　S/Fe　molar　ratios　(S/Fe),　initial　pH　and　dosages　on　16S　rRNA　and　ARGs　removal　were　also　investigated.　Characterization,　mass　balance　and　scavenging　experiments　were　conducted　to　explore　the　mechanisms　of　the　gene　removal.　Quantitative　PCR　(qPCR)　and　high　throughput　fluorescence　qPCR　showed　more　than　3　log　unit　of　16S　rRNA　and　seven　out　of　10　ARGs　existed　in　secondary　effluent　could　be　removed　after　S-nZVI　treatment.　The　mechanisms　might　be　that　DNA　accepted　the　electron　provided　by　the　Fe-0　core　of　S-nZVI　after　being　adsorbed　onto　S-nZVI　surface,　causing　the　decrease　of　16S　rRNA,　ARGs　and　lost　their　regrowth　capacity,　especially　for　typical　MGE　(intI1)　and　further　inhibiting　the　vertical　gene　transfer　(VGT)　and　intI1-induced　horizontal　gene　transfer　(HGT).　Fe-0　core　was　oxidized　to　iron　oxides　and　hydroxides　at　the　same　time.　High　throughput　sequencing,　network　analysis　and　variation　partitioning　analysis　revealed　the　complex　correlations　between　bacteria　and　ARGs　in　secondary　effluent,　S/Fe　could　directly　influence　ARGs　variations,　and　bacterial　genera　made　the　greatest　contribution　to　ARGs　variations,　followed　by　MGEs　and　operational　parameters.　As　a　result,　S-nZVI　could　be　an　available　reductive　approach　to　deal　with　bacteria　and　ARGs.　(C)　2020　Elsevier　Ltd.　All　rights　reserved.