Lymphoma　is　a　heterogeneous　group　of　malignancies　of　the　lymphoid　tissue,　and　is　prevalent　worldwide　affecting　both　children　and　adults　with　a　high　mortality　rate.　There　is　in　dire　need　of　accurate　and　noninvasive　approaches　for　early　detection　of　the　disease.　Herein,　we　report　a　facile　way　to　fabricate　silver　nanoparticle　based　nanoprobe　by　incorporating　the　corner-stone　immunotherapeutic　drug　Rituxan　for　simultaneous　detection　and　ablation　of　lymphoma　cells　in　vitro.　The　fabricated　nanoprobe　can　detect　CD20　positive　single　lymphoma　cell　by　surface　enhanced　Raman　scattering　technique　with　high　specificity.　The　engineered　nanoprobe　retains　the　same　antibody　property　as　intact　drug　via　Antibody-Dependent　Cell-mediated　Cytotoxicity　(ADCC)　analysis.　The　nanoprobe　efficiently　eradicates　lymphoma　cells　in　vitro.　By　integrating　the　advantages　of　sensitive　SERS　detection　with　targeted　ablation　capabilities　of　immunotherapeutic　drug　through　site　specificity,　this　nanoprobe　can　be　applied　as　outstanding　tools　in　living　imaging,　cancer　diagnosis　and　treatment.