Hollow　magnetic　mesoporous　SiO2/Fe3O4　microspheres　with　different　mesopore　sizes　were　synthesized　by　a　simple　multi-step　solution　growth　process.　In　the　experiment　of　coating　mesoporous　SiO2,　cetyltrimethylammonium　bromide　(CTAB)　was　used　as　surfactant　and　two　swelling　agents,　decane　and　1,　3,　5-　trimethylbenzene　(TMB),　were　used　as　swelling　agents　alone　or　together　with　different　sequence.　The　results　showed　that　the　magnetic　mesoporous　SiO2/Fe3O4　microspheres　have　a　saturation　magnetization　of　13.6　emu-g(-1)　and　a　low　coercivity　(50　Oe).　Different　adding　form　of　swelling　agents　had　great　effect　on　the　microstructure　of　mesoporous　SiO2/Fe3O4　microspheres.　The　mesoporous　SiO2/Fe3O4　microspheres　obtained　by　using　two　swelling　agents　together　had　better　mesoporous　properties.　The　mesoporous　SiO2/Fe3O4　microspheres　exhibited　high　surface　area　(850　m(2).g(-1)),　large　peak　pore　(3.11　nm),　high　pore　volume　(1.23　cm(3).g(-1)).　Moreover,　the　composite　microspheres　had　larger　adsorption　amount　(276　mg/g)　and　quicker　adsorption　rate　(0.178　g/(mg.h))　of　laccase.