BST-2,　also　known　as　CD317,　HM1.24,　or　tetherin,　has　been　recognized　as　a　broad-spectrum　antiviral　molecule　against　the　release　of　enveloped　viruses.　To　observe　the　expression　of　BST-2　in　eukaryotic　cells,　BST-2　cDNA　was　amplified　by　PCR,　and　then　cloned　into　the　eukaryotic　expression　vector　pEGFP-C3　or　pFC15A　individually.　The　recombinated　constructs　were　transfected　into　293　FT　cells.　Under　the　fluorescent　microscope　and　confocal　laser　scanning　microscope,　the　BST-2　fusion　protein　mainly　distributed　outside　the　nucleus　unevenly.