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The start-up of ANAMMOX bio-filter for domestic secondary effluent was investigated in ambient temperature. The raw water for ANAMMOX bio-filter was the effluent from an anoxic-aerobic (A/O) phosphorous removal system. Nitrification biofilm was successfully transformed into ANAMMOX biofilm during 7 months, with the induction of added nitrite, hydrazine and oxyammonia. The highest TN removal rate reached 6.8 kg/m(3)/d during start-up, with observed growth rate of 0.0018 h(-1) and doubling time of 16.45 d.
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