In　the　anticancer　research　with　traditional　Chinese　medicine,　many　medicinally　effective　components　can　only　dissolve　in　higher　polar　organic　solvents,　such　as　ethanol,　dimethyl　sulfoxide　(DMSO)　etc.　However,　organic　solvents　may　directly　interfere　with　the　accuracy　of　therapeutic　efficacy　evaluation.　Therefore　the　study　on　effects　of　organic　solvents　with　different　concentrations　on　Hela　cells　is　of　great　significance.　The　digital　holography　is　a　non-destructive　and　non-contact　method　to　image　the　transparent　sample　without　staining　and　with　the　high　precision　and　high　resolution.　In　this　paper,　the　digital　holography　is　proposed　to　replace　the　methyl-thiazol-tetrazolium　(MTT)　or　the　Giemsa　dye　method.　Based　on　the　pre-magnification　off-axis　Fresnel　digital　holographic　theory,　an　inverted　microscopy　system　is　built　to　obtain　the　phase-contrast　images　of　the　Hela　cells,　which　are　added　different　concentrations　of　organic　solvents.　Compared　to　the　control　group,　there　is　significantly　differences　with　the　shapes　of　Hela　cells　with　different　organic　solvents.　The　size　of　cell　with　ethanol　25%　is　no　significantly　difference　with　the　control　group.　But　the　sizes　of　cells　in　the　solutions　with　ethanol　12.5%　and　50%　are　smaller　than　the　control　group.　Next,　the　sizes　of　cells　in　the　solutions　with　DMSO　12.5%,　25%　and　50%　are　great　smaller,　compared　with　the　control　group.　The　results　show　that　the　digital　holography　has　high　practical　value　in　detecting　the　changes　in　the　shape　of　cells　and　is　helpful　in　the　choice　of　organic　solvents　for　further　apoptosis　study.