In　this　study,　the　effect　of　environment　materials　for　civil　engineering　was　investigated　through　two　sets　of　parallel　reactors　with　sodium　acetate　and　starch　as　carbon　source　separately.　The　reactors　were　started　up　by　two　stages,　including　biofilm　formation　stage　and　biofilm　domestication　stage.　In　biofilm　domestication　stage,　the　average　removal　efficiencies　of　COD　were　88.84%　and　88.78%　respectively.　NH4+-N　removal　efficiencies　were　reached　at　99.28%　and　91.90%.　Total　nitrogen　(TN)　removal　efficiencies　were　45.25%　and　45.45%.　By　PCR-DGGE,　it　was　found　that　there　were　high　similarities　between　biofilm　and　suspension　in　the　reactors.　Different　types　of　carbon　sources　enriched　different　types　of　microorganisms.　The　study　can　provide　references　for　studying　simultaneous　nitrification　and　denitrification　(SND)　and　optimizing　carbon　sources.