Novel　targets　against　HIV-1　are　booming　recently.　APOBEC3G　has　the　potential　to　inhibit　the　replication　of　HIV-1,　while　its　antiviral　activity　is　counteracted　by　Vif.　The　antiviral　mechanism　of　APOBEC3G　and　the　antagonistic　mechanism　of　Vif　have　drawn　great　attention.　Considering　the　occurrence　of　interaction　between　Vif　and　APOBEC3G　should　be　based　on　that　these　two　proteins　coexist　in　the　same　cells,　a　eukaryotic　bicistronic　vector　was　adopted　to　express　both　Vif　and　APOBEC3G　simultaneously.　The　expression　and　distribution　of　Vif　and　APOBEC3G　proteins　are　detected　with　fluorescent　microscope.