Microchips　appear　to　offer　important　opportunities　for　modern　research.　Microchips　based　on　gold　magnetic　nanoparticles　make　manipulation　of　biomolecules　more　conveniently,　and　has　the　advantage　of　deducing　reaction　time.　In　this　study,　we　first　achieved　microchips　design　and　fabrication　through　CO2　laser　ablation　for　immunoassay,　and　then　conjugated　mouse　IgG　to　gold　magnetic　nanoparticles　serve　as　a　target.　Finally,　HRP　labeled　goat　anti　mouse　IgG　binding　assay　and　substrate　reaction　were　performed　with　disposable　microchips　and　other　devices.　The　results　indicated　that　the　reactions　carried　on　successfully　and　magnetic　particles　moved　well　in　chips.