The　number　of　cells　is　commonly　employed　to　describe　the　cell　viability　and　the　status　of　cell　culture　in　a　certain　extent.　An　automatic　and　non-invasive　detecting　method　for　the　status　analysis　of　cell　culture　is　developed　based　on　digital　holography　microscopy　(DHM)　technology.　Digital　holographic　imaging　can　retrieve　quantitative　information　of　object　wavefront　by　the　numerical　reconstruction　from　a　single　digital　hologram　recorded　by　a　detector　such　as　CCD　or　CMOS　camera,　which　is　especially　suitable　for　the　morphology　detection　of　the　transparent　or　semi-transparent　cells.　In　this　contribution,　the　lensless　Fourier　transform　(LFT)　based　holography　configuration　is　designed　for　cell　imaging　without　prestaining,　and　the　amplitude　and　phase　of　living　cells　can　be　reconstructed　by　digital　reconstruction　and　phase　unwrapped　algorithms.　Then　the　image　filtering　and　segmentation　are　combined　for　the　automatic　evaluation　of　the　level　of　confluency.　In　imaging　experiments,　the　culture　status　of　the　cervical　cancer　cell　TZMbl　is　detected,　and　the　results　demonstrate　that　digital　holography　microscopy　provides　a　feasible　non-invasive　method　for　monitoring　the　living　cell　culture.