The　process　of　dentine　removal　by　ultra-short　pulse　laser　is　a　promising　technique　for　pain-free　dental　restoration.　The　aim　of　this　work　was　to　analyze　the　characteristics　of　human　dentine　ablated　by　a　fs-laser　of　800　nm　wavelength　and　1　kHz　repetition　rate.　Precise　ablation　with　clear　boundary　whereas　without　cracks　and　debris　can　be　obtained.　The　transmittance　of　the　dentine　tissue　was　about　2.7%　and　the　absorption　coefficient　was　about　3.19　cm(-1)　at　800　nm.　The　processed　morphology,　especial　for　heat-affected　zones,　of　the　dentine　was　investigated　over　wide　ranges　of　laser　fluences　and　pulse　numbers.　The　fluence　threshold　results　of　dentine　were　determined　as　0.45　J/cm(2)　according　to　the　experimental　dependence　of　the　ablated　depth　versus　laser　fluence.　The　heat　and　energy　conducting　effects　of　dentine　tubules,　which　is　the　feature　structure,　were　analyzed　by　Scanning　Electron　Microscopy　and　Wyko　Optical　Profiling　System.　The　finding　of　Raman　mapping　analysis　of　phosphate　indicated　that　the　chemical　components　and　structure　of　the　dentine　remained　intact　before　and　after　ablation.　This　study　confirmed　the　potential　of　fs-laser　precision　micro　fabrication　in　dentistry.