The　problem　of　human-structure　dynamic　interaction　need　to　be　considered　when　the　dynamic　responses　of　structure　and　human　occupants　under　ambient　vibration　are　studied.　The　vertical　human　body　models　with　different　postures　are　selected　according　to　ISO5982,　and　the　dynamic　coupling　equations　with　non-classical　damping　for　structure　and　human　are　established.　The　equations　are　decoupled　by　spatial　state　model　method.　A　new　method　for　human　comfort　evaluation　is　presented　by　obtaining　the　frequency-band　energy　with　wavelet　packet　transform　based　on　ISO2631.　A　10-story　building　near　the　light　rail　transit　system　is　studied　to　analyze　the　dynamic　performance　for　different　numbers　of　human　occupants　and　different　body　postures.　The　results　show　that　the　vibration　reduction　effects　of　floors　are　more　obvious　when　the　numbers　of　human　occupants　increase　for　the　structure　with　even　mass　and　stiffness.　The　vibration　reduction　effects　and　human　comfort　decrease　for　higher　floors.　The　discomfort　of　the　seated　human　body　is　most　significant.　The　comfort　evaluation　method　in　this　paper　is　reliable　and　convenient,　which　could　be　used　as　a　supplementary　method.