The　sulfated　Lycium　barbarum　polysaccharide　(LBP)　were　prepared　by　chlorosulfonic　acid-pyridine　(PY-SO(3))　method　according　to　orthogonal　L(9)(3)(4)　test　under　microwave　irradiation.　Eight　sulfated　sLBP,　with　various　degrees　of　sulfate　(DS)　and　the　number　average　molecular　weight　(M(w)),　were　obtained　and　their　anti-HIV　activities　were　compared　by　MTT　and　ELISA　assay.　The　results　indicated　that　when　DS　within　the　scope　of　0.2-0.5　and　the　M(w)　in　the　range　of　40-50　kDa,　sLBP　exhibited　significantly　strong　inhibition　of　HIV　activity,　and　it　was　confirmed　by　real-time　biomolecular　interaction　analysis　(BIAcore).　The　optimum　sulfation　conditions　were　reaction　temperature　of　45　degrees　C,　the　molar　ratio　of　Py-SO(3)　to　LBP　of　2:1　and　the　reaction　time　of　6　min　at　the　microwave　power　of　45W　in　DMF.