In　this　work,　lithium　(Li)-aluminum　(Al)　layered　double　hydroxide　(LDH)　films　modified　by　4-amino-2-((hydrazine　methylene)　amino)-4-oxobutanoic　acid　(denoted　as　AOA　acid)　and/or　1H,1H,2H,2H-perfluorooctyltriethoxysilane　were　prepared　on　6N01　Al　alloy　by　a　facile,　in-situ　growth　method　with　enhanced　hydrophobicity,　anti-biofouling　and　anti-corrosion　performance.　The　preparation　is　low　energy　consumptive　and　environment　friendly,　relying　on　self-assembly　at　ambient　temperature.　The　structure,　molecular　weight　and　functional　groups　of　the　synthesized　AOA　acid　were　characterized　by　NMR　spectrometer,　ESI-MS　spectrometer　and　Fourier　transform　infrared　(FT-IR)　spectroscopy.　And　the　compositions,　structure　and　morphology　of　the　films　were　characterized　by　Fourier　transform　infrared　(FT-IR)　spectroscopy,　glancing-angle　X-ray　diffraction　(GA-XRD),　X-ray　photoelectron　spectroscopy　(XPS),　field　emission　scanning　electron　microscopy　(FE-SEM)　and　energy-dispersive　x-ray　spectrum　(EDS).　Water　contact　angle　measurements　(CA)　and　atomic　force　microscopy　(AFM)　characterization　show　that　the　films　possess　a　micro/nanostructure　with　an　improved　hydrophobicity.　Immersion　test,　neutral　salt　tests　(NSS)　and　electrochemical　impedance　spectroscopy　(EIS)　conducted　in　3.5　wt.%　NaCl　solutions　demonstrate　the　improved　corrosion　resistance　of　the　films　over　bare　Al　alloy.　Meanwhile,　the　films　also　possess　an　excellent　anti-bacterial　property　to　Escherichia　coli,　Bacillus　subtilis　and　sulfate-reducing　bacteria.　(C)　2021　Published　by　Elsevier　Ltd　on　behalf　of　The　editorial　office　of　Journal　of　Materials　Science　&　Technology.