Immunomagnetic　beads　(IMBs)　for　cell　sorting　are　universally　used　in　medical　and　biological　fields.　At　present,　the　IMBs　on　the　market　are　ferrite　coated　with　a　silicon　shell.　Based　on　a　new　type　of　magnetic　material,　the　graphene　coated　iron　nitride　magnetic　particle　(G@FeN-MP),　which　we　previously　reported,　we　prepared　a　novel　IMB,　a　graphene　oxide　coated　iron　nitride　immune　magnetic　bead　(GO@FeN-IMBs),　and　explored　its　feasibility　for　cell　sorting.　First,　the　surface　of　the　G@FeN-MP　was　oxidized　to　produce　oxygen-containing　groups　as　carboxyl,　etc.　by　the　optimized　Hummers＇　method,　followed　by　a　homogenization　procedure　to　make　the　particles　uniform　in　size　and　dispersive.　The　carboxy　groups　generated　were　then　condensed　and　coupled　with　anti-CD3　antibodies　by　the　carbodiimide　method　to　produce　an　anti-CD3-GO@FeN-IMB　after　the　coupling　efficacy　was　proved　by　bovine　serum　albumin　(BSA)　and　labeled　antibodies.　Finally,　the　anti-CD3-GO@FeN-IMBs　were　incubated　with　a　cell　mixture　containing　human　T　cells.　With　the　aid　of　a　magnetic　stand,　the　T　cells　were　successfully　isolated　from　the　cell　mixture.　The　isolated　T　cells　turned　out　to　be　intact　and　could　proliferate　with　the　activation　of　the　IMBs.　The　results　show　that　the　G@FeN-MP　can　be　modified　for　IMB　preparation,　and　the　anti-CD3-GO@FeN-IMBs　we　prepared　can　potentially　separate　T　cells.