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Author:

Liang, Tianya (Liang, Tianya.) | Li, Jianxing (Li, Jianxing.) | Liu, Xiao (Liu, Xiao.) (Scholars:刘晓) | Ma, Zhuang (Ma, Zhuang.) | Su, Xiaojin (Su, Xiaojin.) | Meng, Xiangjiao (Meng, Xiangjiao.) | Zhanghuang, Ziyi (Zhanghuang, Ziyi.) | Wang, Huiqin (Wang, Huiqin.) | Li, Jintao (Li, Jintao.) | Wang, Qun (Wang, Qun.) (Scholars:王群) | Wang, Minglian (Wang, Minglian.)

Indexed by:

Scopus SCIE

Abstract:

Immunomagnetic beads (IMBs) for cell sorting are universally used in medical and biological fields. At present, the IMBs on the market are ferrite coated with a silicon shell. Based on a new type of magnetic material, the graphene coated iron nitride magnetic particle (G@FeN-MP), which we previously reported, we prepared a novel IMB, a graphene oxide coated iron nitride immune magnetic bead (GO@FeN-IMBs), and explored its feasibility for cell sorting. First, the surface of the G@FeN-MP was oxidized to produce oxygen-containing groups as carboxyl, etc. by the optimized Hummers' method, followed by a homogenization procedure to make the particles uniform in size and dispersive. The carboxy groups generated were then condensed and coupled with anti-CD3 antibodies by the carbodiimide method to produce an anti-CD3-GO@FeN-IMB after the coupling efficacy was proved by bovine serum albumin (BSA) and labeled antibodies. Finally, the anti-CD3-GO@FeN-IMBs were incubated with a cell mixture containing human T cells. With the aid of a magnetic stand, the T cells were successfully isolated from the cell mixture. The isolated T cells turned out to be intact and could proliferate with the activation of the IMBs. The results show that the G@FeN-MP can be modified for IMB preparation, and the anti-CD3-GO@FeN-IMBs we prepared can potentially separate T cells.

Keyword:

immune magnetic beads graphene oxide iron nitride magnetic particles T cell separation

Author Community:

  • [ 1 ] [Liang, Tianya]Beijing Univ Technol, Fac Environm & Life Sci, Beijing 100124, Peoples R China
  • [ 2 ] [Li, Jianxing]Beijing Univ Technol, Fac Environm & Life Sci, Beijing 100124, Peoples R China
  • [ 3 ] [Su, Xiaojin]Beijing Univ Technol, Fac Environm & Life Sci, Beijing 100124, Peoples R China
  • [ 4 ] [Meng, Xiangjiao]Beijing Univ Technol, Fac Environm & Life Sci, Beijing 100124, Peoples R China
  • [ 5 ] [Zhanghuang, Ziyi]Beijing Univ Technol, Fac Environm & Life Sci, Beijing 100124, Peoples R China
  • [ 6 ] [Wang, Huiqin]Beijing Univ Technol, Fac Environm & Life Sci, Beijing 100124, Peoples R China
  • [ 7 ] [Li, Jintao]Beijing Univ Technol, Fac Environm & Life Sci, Beijing 100124, Peoples R China
  • [ 8 ] [Wang, Minglian]Beijing Univ Technol, Fac Environm & Life Sci, Beijing 100124, Peoples R China
  • [ 9 ] [Liu, Xiao]Beijing Univ Technol, Fac Mat & Mfg Sci, Beijing 100124, Peoples R China
  • [ 10 ] [Ma, Zhuang]Beijing Univ Technol, Fac Mat & Mfg Sci, Beijing 100124, Peoples R China
  • [ 11 ] [Wang, Qun]Beijing Univ Technol, Fac Mat & Mfg Sci, Beijing 100124, Peoples R China

Reprint Author's Address:

  • 王群

    [Wang, Minglian]Beijing Univ Technol, Fac Environm & Life Sci, Beijing 100124, Peoples R China;;[Wang, Qun]Beijing Univ Technol, Fac Mat & Mfg Sci, Beijing 100124, Peoples R China

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Source :

MAGNETOCHEMISTRY

Year: 2021

Issue: 5

Volume: 7

2 . 7 0 0

JCR@2022

JCR Journal Grade:2

Cited Count:

WoS CC Cited Count: 1

SCOPUS Cited Count: 2

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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