This　work　investigated　the　feasibility　of　a　novel　simultaneous　enhanced　biological　phosphorus　removal　and　semi-nitritation　(EBPR-SN)　plus　anammox　process　treating　real　municipal　wastewater　from　summer　to　winter　(28.1- 15.3 °C).　Two　lab-scale　sequential　reactors　were　used　in　this　study,　namely　EBPR-SN　and　Anammox　sequencing　batch　reactors　(SBRs).　Long-term　operation　suggested　that　ammonium　oxidizing　bacteria　abundance　decreased　from　1.67%　to　0.89%　whereas　nitrite　oxidizing　bacteria　decreased　to　nearly　undetected　in　the　EBPR-SN　SBR,　maintaining　the　stable　nitritation　(nitrite　accumulation　ratio:　98.3 ± 1.0%).　Lowering　airflow　rate　was　effective　to　retain　nitritation　with　temperature　decrease.　Reliable　nutrient　removal　was　still　maintained　in　winter　(16.4 ± 0.7 °C),　i.e.　the　removal　efficiencies　for　nitrogen　and　phosphorus　were　80.0 ± 3.5%　and　95.4 ± 5.2%,　respectively,　with　short　aerobic　HRT　(6.4 h)　and　low　dissolved　oxygen　(0.2-1.5 mg/L).　The　percentage　of　anammox　contribution　to　nitrogen-removal　increased　with　temperature　decrease,　although　Candidatus　Brocadia　abundance　decreased.　Additionally,　the　protection　of　extracellular　polymeric　substances　was　important　to　the　successful　performance.