As　a　unique　characteristic　two-dimensional　transition　metal　dichalcogenide,　molybdenum　disulfide　(MoS2)　has　become　a　promising　membrane　material　for　organic　solvent　nanofiltration　(OSN)　due　to　its　good　separation　performance　and　stability.　In　this　work,　an　MoS2　tubular　ceramic　membrane　is　prepared　via　an　in　situ　hydrothermal　method,　exhibiting　quite　good　stability　in　organic　solvent.　The　3D　nanoflower-like　structure　of　the　MoS2　layer　endows　the　modified　membrane　with　an　excellent　OSN　performance.　With　separation　of　a　methanol　solution　of　Evans　blue　(EB),　the　methanol　flux　was　403.1　L　m(-2)　h(-1)　MPa-1　and　the　rejection　of　EB　was　98.1%.　The　adhesion　structures　at　the　surface　of　MoS2/Al2O3　were　evaluated　using　density　functional　theory,　and　an　interfacial　distance　of　3.2　angstrom　was　obtained.　Considering　non-bond　S-O　interactions　in　addition　to　van　der　Waals　forces,　the　work　of　adhesion　(0.55　J　m(-2))　and　charge　transfer　process　between　the　MoS2　layer　and　the　ceramic　substrate　indicate　good　stability.　The　MoS2　tubular　ceramic　membrane　maintains　separation　stability　after　performing　in　organic　solvents　of　DMF　for　80　h　under　cross-flow.