In　this　experiment,　the　effects　of　different　stirring　methods　on　the　rejuvenation　and　operation　of　anaerobic　ammonium　oxidation　sludge　under　normal　temperature　(21±1)　conditions　were　investigated.　Three　groups　of　reactors　(R1,　R2,　and　R3)　were　studied,　and　the　stirring　methods　were　continuous　stirring　for　R1　and　intermittent　stirring　with　different　rotation　speeds　for　R2　and　R3.　The　denitrification　performance　and　the　performance　of　granular　sludge　during　system　rejuvenation　and　operation　process　were　analyzed.　Results　show　that　the　removal　rates　of　ammonia　nitrogen　and　nitrite　nitrogen　of　R1,　R2,　and　R3　were　close　to　100%　in　10,　2　and　2　d,　respectively.　The　anaerobic　ammonium　oxidation　rates　of　R2　and　R3　were　higher,　which　were　more　suitable　for　the　growth　of　anammox　bacteria.　According　to　the　results　of　cycle　test,　the　fluidization　state　of　the　water　flow　formed　by　the　intermittent　stirring　method　was　weak,　so　that　the　reactor　had　lower　dissolved　oxygen　for　a　longer　period,　and　it　was　easier　to　inhibit　the　activity　of　AOB,　thereby　increasing　the　activity　of　the　anammox　bacteria.　The　R1　system　provided　strong　shearing　force　and　had　continuous　contact　with　the　substrate　solution,　resulting　in　more　EPS.　At　the　end　of　the　static　operation　phase,　the　average　particle　size　of　granular　sludge　in　R1,　R2,　and　R3　was　723,　675　and　649　μm,　respectively.　Copyright　©2021　Journal　of　Harbin　Institute　of　Technology.All　rights　reserved.