C-reactive　protein　(CRP)　is　an　acute　phase　reactant,　and　is　an　important　biomarker　whose　concentration　in　serum　can　be　used　for　monitoring　inflammation　and　predicting　atherosclerotic,　cardiovascular　events　etc.　In　this　study,　surface　plasma　resonance(SPR)　technology　is　adopted　in　combination　with　immunoassay　for　CRP　concentration　analysis　and　dynamic　observation　in　limited　time　frame,　thereby　eliminating　complicated　labeling　procedure　and　greatly　reduce　the　complexity　of　the　detection　system.　With　corresponding　fluid　manipulation　enabled　by　a　microfluidic　platform,　this　detection　strategy　holds　the　promise　of　being　a　point-of-care　diagnostic　approach.