In　recent　years,　customized　bus　(CB)　is　becoming　an　innovative　model　of　the　public　transport　system　(PTS)　in　China.　This　service　provides　an　advanced,　timely,　personalized,　and　flexible　responses　to　the　demand　of　the　PTS,　especially　of　commuters.　Obviously,　it　could　have　an　impact　on　public　transport　(PT)　service.　For　instance,　CB　services　influence　the　customers＇　variety-seeking　behavior,　which　results　in　changes　of　customer　options　in　different　periods.　Therefore,　customers　could　be　divided　into　different　groups　of　preference.　In　this　paper,　we　construct　a　two-period　Hotelling　game　model　by　variety-seeking　behavior　and　service　level.　The　results　show　that,　in　the　first　stage,　the　departure　frequency　of　CB　and　PT　is　influenced　by　variety-seeking　behavior　and　service　level.　In　the　second　stage,　departure　frequency　is　influenced　by　service　level　only.　Next,　although　the　departure　frequency　of　bus　impacts　the　changes　of　passenger　ratios　in　different　stages,　service　level　appreciably　impacts　passenger　number.　From　customer　surplus　and　social　welfare,　we　find　that　adjustment　of　service　level　can　increase　social　welfare,　which　means　that　CB　and　PT　will　reach　an　optimum　effect.　The　present　work　can　provide　a　valuable　reference　to　policymakers,　practitioners,　and　others.