A　pilot-scale　two-stage　tidal　flow　constructed　wetland　(TFCW)　with　working　volume　of　0.46　m(3)/d　packing　with　shale　ceramsite　(SC)　and　activated　alumina　(AA)　was　constructed　(named　as　SC-AA-TFCW)　for　nutrients　removal　at　low　temperature　(＜15　degrees　C).　SC-AA-TFCW　achieved　stable　removals　of　78.1%　nitrogen　and　98.3%　phosphorous.　SC-TFCW　contributed　to　55.2%　of　organics　and　85.6%　of　particulate　phosphorous　removal.　Among　17　denitrifiers,　the　absolute　abundance　of　aerobic　denitrification　bacteria　(ADNB)　was　highest,　followed　by　facultative　anaerobic　denitrification　bacteria　(FADNB)　and　autotrophic　denitrification　bacteria　(AUDNB).　Nitrogen　assimilating　into　organic　nitrogen,　dissimilatory　and　assimilatory　nitrate　reduction　and　complete　denitrification　may　be　main　nitrogen　metabolic　pathways.　Some　ADNB　(e.　g.　Zoogloea,　Pseudomonas　and　Acidovorax)　showed　positive　interactions　with　various　key　functional　genes　related　to　nutrients　removal.　Dissolved　oxygen　and　reducing　elements　were　main　environmental　factors　in　changing　ADNB　compositions.　This　study　highlights　the　importance　of　ADNB　and　their　synergy　to　porous　substrates　in　SC-AA-TFCW.