The　dynamic　response　mechanism　of　Candidatus　Accumulibacter　clades　to　environmental　factors　in　enhanced　biological　phosphorus　removal　(EBPR)　was　unclear.　This　study　investigated　the　relationship　between　the　transcriptional　responses　of　Candidatus　Accumulibacter　clades　and　environmental　dynamics.　Results　suggested　that　Candidatus　Accumulibacter　clade　IIA　only　responded　in　initial　20　and　30　min　of　P-release　and　P-uptake　stage,　respectively,　and　was　also　the　first　clade　to　stop　responding　among　the　six　Candidatus　Accumulibacter　clades.　Clade　IIC　and　IID　responded　at　rising　stage　of　P-release　and　P-uptake　rate.　Clade　IA　and　IIB　responded　at　decreasing　stage　of　P-release　and　P-uptake　rate.　The　transcriptional　response　duration　of　clade　IIF　was　the　longest,　which　constantly　responded　throughout　anaerobic,　anoxic　and　oxic　phase.　The　transcriptional　responses　of　Candidatus　Accumulibacter　clades　to　environmental　dynamics　revealed　the　microorganisms　actually　working　in　P-release　and　P-uptake,　and　gave　a　new　insight　into　the　transcriptional　responses　related　to　the　EBPR　performance.