This　study　systematically　investigated　the　fouling　behavior　and　mechanism　of　PAN　Microfiltration　(MF)　membrane　fouled　with　the　model　binary　mixture　(bovine　serum　albumin　(BSA)　and　sodium　alginate　(SA))　of　extracellular　polymer　substance　(EPS)　solution　under　different　concentration　and　transmembrane　pressure　(TMP).　The　result　showed　that　the　fouling　mechanism　of　the　model　binary　mixture　was　the　pore　blocking　and　cake　formation.　Moreover,　the　SA　plays　a　dominant　role　in　the　model　binary　mixture　(BSA/SA).　The　higher　SA　concentration　could　cause　the　more　serious　membrane　fouling　(both　the　flux　(J)　and　the　available　membrane　area　(A/A0)　decreased),　and　the　total　resistance　increased　and　caused　the　fouling　mechanism　changing　from　combined　complete　blocking　and　cake　filtration　into　individual　cake　filtration　appeared　earlier.　In　addition,　increasing　of　the　TMP　can　also　aggravate　membrane　fouling　and　it　could　be　accurately　described　by　the　cake-complete　model.　This　studied　offered　insight　into　the　model　binary　mixture　in　the　fouling　behavior　and　mechanism,　which　can　be　used　as　guidance　for　practical　application.　©　Content　from　this　work　may　be　used　under　the　terms　of　the　Creative　Commons　Attribution　3.0　licence.