We　present　the　first　report　on　automated　micro-manipulation　of　zebrafish　embryos　using　an　electrothermally-actuated　microgripper.　A　five-bar　linkage　compliant　microgripper,　driven　by　a　V-shaped　electrothermal　micro-actuator,　is　designed　based　on　topological　optimization.　The　electrothermally-actuated　microgripper　is　fabricated,　tested,　and　then　integrated　into　a　robotic　micromanipulation　system.　The　semi-automated　manipulation　of　a　single　zebrafish　embryo,　as　well　as　a　series　of　comparison　experiments,　is　carried　out.　Experimental　results　demonstrate　that　the　microgripper　has　reliable　capability　of　picking,　moving,　holding　and　releasing　the　zebrafish　embryo.　Specifically,　it　performs　fairly　well　in　immobilizing　and　limiting　the　excessive　deformation　of　the　embryo.　Finally,　based　on　teaching,　the　whole　process　of　zebrafish　embryo　microinjection　is　programed　and　performed　automatically,　thus　demonstrating　great　strengths　and　performance　of　the　microgripper　in　zebrafish　embryo　micro-manipulation.