The　mammalian　APOBEC3G　protein　(apolipoprotein　B　mRNA-editing　enzyme　catalytic　polypeptide　3　protein　G,　APOBEC3G)　is　an　important　component　of　the　cellular　innate　immune　response　to　retroviral　infection.　APOBEC3G　can　extinguish　HIV-1　(human　immunodeficiency　virus　type　1)　infectivity　by　its　incorporation　into　virus　particles　and　subsequent　cytosine　deaminase　activity　to　block　replication　of　HIV-1.　HIV-1　Vif　(viral　infectivity　factor)　suppresses　various　APOBEC3　proteins　through　a　common　mechanism　which　induces　the　degradation　of　target　proteins.　Therefore,　the　interrelation　of　Vif-APOBEC3G　has　been　extensively　studied,　which　represents　attractive　targets　for　the　development　of　novel　inhibitors.　We　summarize　the　papers　in　which　the　detection　technique　and　methods　have　been　developed　to　assay　the　anti-HIV　activity　and　its　mechanism,　such　as　western-blotting,　co-immunoprecipitation,　pulse-chase　experiments,　bioluminescence　resonance　energy　transfer,　biomolecular　interaction　analysis.　This　review　is　towards　developing　therapeutics　aimed　at　the　Vif-APOBEC3G　axis.