To　study　the　effect　of　anti-HIV　III　B　virus　with　extractions　from　Juglans　regia,　so　as　to　searching　for　the　new　and　efficacious　leading　compound　of　AIDS　therapy.Phytochemical　and　chromatographical　techniques　were　used　to　isolate　compounds　from　J.　regia;　MT4　cells　and　HIV　III　B　virus　were　used　to　study　the　effect　of　anti-HIV　activity　in　vitro.　BIACORE　3000　molecule　coupled　equipment　were　used　for　the　target　research　also.Two　extractions　(B&E)　were　isolated　from　J.　regia　which　possess　the　effect　of　anti-HIV　activity.　Targets　study　found　that　extraction　B　could　affected　on　HIV-1　gp-41　fusing　protein　and　extraction　E　could　affected　on　HIV-1　integrase　respectively.J.　regia　is　a　traditional　Chinese　medicine　with　active　anti-HIV　activity　and　worth　to　be　studied　further.