Apolipoprotein　B　mRNA-editing　enzyme　catalytic　polypeptide-like　3G　(APOBEC3G)　cDNA　was　amplified　from　total　RNA　prepared　from　nonpermissive　H9　cells　by　RT-PCR.　APOBEC3G　cDNA　is　1155nt　long,　encoding　384　amino　acids.　The　APOBEC3G　gene　was　then　cloned　into　the　eukaryotic　expression　vector　pEGFP-C3.　The　generated　pEGFP-3G　construct　was　then　transfected　into　CD4+　HeLa　cell　to　determine　the　expression　and　the　subcellular　localization　of　GFP-APOBEC3G　fusion　protein.　Under　CLSM　the　localization　of　the　expressed　GFP-APOBEC3G　in　the　cytoplasm　of　CD4+　HeLa　cells　was　observed.