The　invention　belongs　to　the　field　of　pharmaceutical　chemistry,　　and　provides　an　application　of　virtual　screening　in　the　preparation　of　a　protein　kinase　inhibitor　and　a　drug　lead　compound,　　said　screening　method　comprising　the　following　steps　:　　(1)　obtaining　a　candidate　compound　by　means　of　virtual　screening;　　(2)　testing　the　in　vitro　protein　kinase　activity　and　anti-tumor　cell　proliferation　activity　of　the　candidate　compound　obtained　in　step　(1),　　and　screening　to　obtain　a　target　compound.　The　screening　method　obtains　a　target　compound　which　has　inhibitory　activity　on　CK2　and　lung　cancer　cells　A549,　　and　the　method　has　such　advantages　as　being　time-saving,　　effort-saving,　　cost-saving,　　and　high-efficiency.