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Improving contribution and nitrogen removal efficiency (NRE) of partial anammox in municipal wastewater is a researching hotspot. This study developed an innovative PN/A-PD/A process with fixed biocarriers in anaerobic/anoxic chambers for actual sewage treatment in a typical step-feed reactor over 390 days. Two coupled pathways providing continuous NO2- (partial nitrification in oxic chambers and partial denitrification in anaerobic/anoxic chambers) for anammox were introduced to the process, achieving 47% nitrogen loss by anammox in stable phase. The influent and effluent total inorganic nitrogen (TIN) were 51.3 and 11.0 mg/L, respectively, even with chemical oxygen demand (COD)/TIN ratio of 2.9. Anammox activity improved from 6.52 to 9.68 mg NH4+-N/gMLSS/d and abundance on the biocarriers raised to 3.16 x 10(10) gene copies/g dry sludge. Overall, this study confirmed partial anammox, spatially coupled with partial nitrification and partial denitrification via oxic/anoxic distribution with step feed mode, as an alternative for application of mainstream anammox.
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